<i>Phaseolus vulgaris MIR1511</i> genotypic variations differentially regulate plant tolerance to aluminum toxicity

نویسندگان

چکیده

The common-bean (Phaseolus vulgaris), a widely consumed legume, originated in Mesoamerica and expanded to South America, resulting the development of two geographically distinct gene pools. Poor soil condition, including metal toxicity, are often constraints crop production. Several P. vulgaris miRNAs, miR1511, respond toxicity. MIR1511 sequence from model sequenced genotypes revealed that, as opposed BAT93 (Mesoamerican), G19833 (Andean) accession displays 58-bp deletion, comprising mature star miR1511 sequences. Genotyping-By-Sequencing data analysis 87 non-admixed Phaseolus genotypes, different species populations, that all Andean part Mesoamerican (MW1) analyzed displayed truncated gene. geographic origin with complete versus showed distribution. ALS3 (Aluminum Sensitive Protein 3) gene, known be important for aluminum detoxification several plants, was experimentally validated target. Roots plants decreased increased transcript levels at early stages under toxicity (AlT), while lacking higher earlier response. Root architecture analyses evidenced tolerance AlT. However, engineered overexpression lower level sensitivity Absence diminished degradation, appears an evolutionary advantage high Al soils drought conditions.

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ژورنال

عنوان ژورنال: Plant Journal

سال: 2021

ISSN: ['1365-313X', '0960-7412']

DOI: https://doi.org/10.1111/tpj.15129